Relationship between Acylsugars and Leaf Trichomes: Mediators of Pest Resistance in Tomato.

Tomato plants are highly susceptible to pests. Among the control methods, genetic improvement with introgression of resistance genes from wild accessions into commercial tomato lines is the best alternative for an integrated pest management (IPM). Thus, the objective of this study was to select tomato genotypes in advanced populations (F2BC3), with higher levels of acylsugar content, greater recurrent parent genome recovery, and resistance to Tetranychus urticae and Bemisia tabaci inherited from Solanum pennellii. For pest resistance, bioassays were assessed: nine high-acylsugar genotypes, four low-acylsugar genotypes, and the parents, Solanum lycopersicum or 'Redenção', and Solanum pennellii LA-716. Glandular and non-glandular trichomes were quantified. A negative correlation was measured between acylsugar content in the leaflets and pest behavior. Pest resistance was found in the selected F2BC3 genotypes with high-acylsugar content, indicating that this allelochemical was efficient in controlling the arthropod pests.

Morphology of immature stages, biological parameters and life table of Microtechnites bractatus (Hemiptera: Miridae) on different host plants

ABSTRACT The garden fleahopper, Microtechnites bractatus (Say) (Hemiptera: Miridae), is associated with several cultivated plant species and, despite its economic importance, little is known about its development and performance in such hosts. We described here, the morphology of immature stages, and evaluated the biology of M. bractatus in beans, potatoes, white clover, alfalfa, and wheat. The bioassays were carried out in the laboratory under controlled temperature (25±2ºC), humidity (UR70±15%), and photoperiod (12L:12D). The eggs of M. bractatus are elongated and slightly curved, without respiratory projections and light yellow in color, becoming dark at the end of the incubation period. Nymphs present an oval-shaped body, a reddish color that intensifies along with the development and dimorphic wing pads in the fifth instar. In the bioassays, the host plants influenced the biological aspects of M. bractatus, both in the immature and adult stages. Nymph survival was higher in clover and alfalfa, while in wheat, it was lower. Clover-fed insects had the longest longevity. The fecundity parameters and egg viability were favored in insects that fed on clover and alfalfa. The fertility life table showed that feeding M. bractatus with clover provides a higher net reproduction rate (R0) and a higher finite ratio of population increase (ʎ). This study contributes to bioecological and behavioral studies on M. bractatus and provides data for the recognition and characterization of individuals in the immature stage.

Feeding Behavior of Collaria scenica (Stål) (Hemiptera: Miridae) on Wheat Plants: an EPG Waveform Characterization.

In this study, we use for the first time the electropenetrography (EPG) technique to characterize and describe the feeding activities of Collaria scenica (Stål) adults and their respective waveforms on wheat, Triticum aestivum L. plants. It comprised of four waveforms, two related to non-feeding (Np and R) and two with feeding activities (CR and I). The Np wave represents the insect resting or walking on the plant surface (75% of the recording time). R wave was associated to sensory evaluation of leaf surface with the tip of the labium (labial dabbing activity-ca. 1% of the recording time). Feeding activities were of relative short duration; cell rupture waveform (CR-ca. 49 min-ca. 15% of the recording time) was highly irregular due to constant movement of the stylets internally in the plant tissue. The waveform I (ca. 28 min-ca. 9% of the recording time) showed stereotypical and repetitive pattern and represents ingestion of the cell contents previously degraded via cell-rupturing activities. The damage caused by C. scenica in wheat leaflets is a result of cell-rupturing activities, generated by action of digestive enzymes plus mechanical action of serrated mandibular stylets. This results in whitish streaks, dots, or spots on the leaflet of wheat plants which cause significant damage and eventually plants' death.

Sexual dimorphism in Diabrotica speciosa and Diabrotica viridula (Coleoptera: Chrysomelidae)

ABSTRACT Diabrotica speciosa (Germar) and Diabrotica viridula F. (Coleoptera: Chrysomelidae) are the two most abundant species of the genus in South America, and belong to the fucata and virgifera groups, respectively. Here, we characterize the dimorphism of the setae present on the basitarsi of males and females of these species. Dimorphism was confirmed in both species, and it was related to the presence of adhesive setae exclusively in males, which possess these structures on the basal tarsomeres of the pro- and mesothoracic legs.

Trichomes and Allelochemicals in Tomato Genotypes Have Antagonistic Effects Upon Behavior and Biology of Tetranychus urticae.

Tomato genotypes selected for their high foliar zingiberene (ZGB) contents in a segregating F2 population were assessed to determine their effect on behavior and biology of Tetranychus urticae Koch, the putative resistance mechanisms involved and the role of trichomes on that resistance. Genotypes with contrasting ZGB content (RVTZ-09 = low ZGB, RVTZ-79 = high ZGB, RVTZ-142 = high ZGB, and RVTZ-331 = high ZGB) were selected from an interspecific cross between wild S. habrochaites var. hirsutum accession PI-127826 (high ZGB content and resistant to mites) and S. lycopersicum cv. Redenção (low ZGB content and susceptible to mites). To determine the effect of these genotypes on mite behavior and biology, free- and no-choice tests, as well as biological studies were performed. Types and densities of trichomes on the foliar surface and their correlation with ZGB contents was determined. Genotypes rich in ZGB (RVTZ-79, RVTZ-142, and RVTZ-331) presented a high number of types IV and VI glandular trichomes, and both type IV and VI densities were positively correlated with ZGB content. In the free-choice test, T. urticae showed a high preference toward S. lycopersicum cv. Redenção and the genotype RVTZ-09 (low ZGB content), whereas, genotypes with high ZBG content were less preferred. Moreover, on high ZGB genotypes, increase in the egg incubation period and in total mortality of nymphs, and decrease of fecundity rate were observed, indicating deleterious effects in mite biology. Results indicated that high ZGB/high glandular trichome densities genotypes present both non-preference and antibiosis mechanisms of resistance to the mite.

A novel interaction between plant-beneficial rhizobacteria and roots: colonization induces corn resistance against the root herbivore Diabrotica speciosa.

A number of soil-borne microorganisms, such as mycorrhizal fungi and rhizobacteria, establish mutualistic interactions with plants, which can indirectly affect other organisms. Knowledge of the plant-mediated effects of mutualistic microorganisms is limited to aboveground insects, whereas there is little understanding of what role beneficial soil bacteria may play in plant defense against root herbivory. Here, we establish that colonization by the beneficial rhizobacterium Azospirillum brasilense affects the host selection and performance of the insect Diabrotica speciosa. Root larvae preferentially orient toward the roots of non-inoculated plants versus inoculated roots and gain less weight when feeding on inoculated plants. As inoculation by A. brasilense induces higher emissions of (E)-ß-caryophyllene compared with non-inoculated plants, it is plausible that the non-preference of D. speciosa for inoculated plants is related to this sesquiterpene, which is well known to mediate belowground insect-plant interactions. To the best of our knowledge, this is the first study showing that a beneficial rhizobacterium inoculant indirectly alters belowground plant-insect interactions. The role of A. brasilense as part of an integrative pest management (IPM) program for the protection of corn against the South American corn rootworm, D. speciosa, is considered.

Weather forecasting by insects: modified sexual behaviour in response to atmospheric pressure changes.

Prevailing abiotic conditions may positively or negatively impact insects at both the individual and population levels. For example while moderate rainfall and wind velocity may provide conditions that favour development, as well as movement within and between habitats, high winds and heavy rains can significantly decrease life expectancy. There is some evidence that insects adjust their behaviours associated with flight, mating and foraging in response to changes in barometric pressure. We studied changes in different mating behaviours of three taxonomically unrelated insects, the curcurbit beetle, Diabrotica speciosa (Coleoptera), the true armyworm moth, Pseudaletia unipuncta (Lepidoptera) and the potato aphid, Macrosiphum euphorbiae (Hemiptera), when subjected to natural or experimentally manipulated changes in atmospheric pressure. In response to decreasing barometric pressure, male beetles exhibited decreased locomotory activity in a Y-tube olfactometer with female pheromone extracts. However, when placed in close proximity to females, they exhibited reduced courtship sequences and the precopulatory period. Under the same situations, females of the true armyworm and the potato aphid exhibited significantly reduced calling behaviour. Neither the movement of male beetles nor the calling of armyworm females differed between stable and increasing atmospheric pressure conditions. However, in the case of the armyworm there was a significant decrease in the incidence of mating under rising atmospheric conditions, suggesting an effect on male behaviour. When atmospheric pressure rose, very few M. euphorbiae oviparae called. This was similar to the situation observed under decreasing conditions, and consequently very little mating was observed in this species except under stable conditions. All species exhibited behavioural modifications, but there were interspecific differences related to size-related flight ability and the diel periodicity of mating activity. We postulate that the observed behavioral modifications, especially under decreasing barometric pressure would reduce the probability of injury or death under adverse weather conditions.

[Population dynamics and vertical distribution of Scaptocoris carvalhoi Becker (Hemiptera: Cydnidae) in pastures]. / Flutuação populacional e distribuição vertical de Scaptocoris carvalhoi Becker (Hemiptera: Cydnidae) em área de pastagem.

Studies on population dynamics and vertical distribution in the soil of Scaptocoris carvalhoi Becker were carried out in a pasture area in Paraúna, State of Goias, Brazil, from November 2004 to October 2005. One meter-deep holes were excavated in the soil, except on the season of lower precipitation, when 2-meter holes were excavated (June, August and September 2005). On each evaluation, the specimens were collected and grouped according to the depth they were found in the soil profile (0-20, 21-40, 41-60, 61-80 and 81-100 cm). Nymphs occurred during the entire length of the study and were more abundant than adults. Adult population augmented with the increase of rainfall. Nymphs and adults were located in the top layer of the soil during the rainy season and were found deeper in the soil during droughts. However, adults were less tolerant to hydric stress and burrowed deeper into the soil at the beginning of the dry season, while nymphs burrowed deeper only at the end of the season. Results of this study are in accordance to other studies on various species of Scaptocoris, in other regions of Brazil.

Flutuação populacional e distribuição vertical de Scaptocoris carvalhoi Becker (Hemiptera: Cydnidae) em área de pastagem / Population dynamics and vertical distribution of Scaptocoris carvalhoi Becker (Hemiptera: Cydnidae) in pastures

Os estudos de levantamento populacional e distribuição vertical de Scaptocoris carvalhoi Becker foram realizados em área de pastagem de Paraúna, GO, entre os meses de novembro de 2004 e outubro de 2005. Em cada avaliação foram realizadas escavações de 1 m de profundidade, coletando os indivíduos e agrupando-os de acordo com a profundidade em que se localizavam no perfil do solo (0-20, 21-40, 41-60, 61-80 e 81-100 cm). Quando os insetos foram encontrados em quantidades baixas até 1 m, as escavações foram realizadas até 2 m de profundidade (junho, agosto e setembro de 2005). As ninfas foram predominantes durante todo o período. O número de adultos foi dependente das chuvas, constatando-se maior incidência com o incremento da precipitação. Ninfas e adultos mantiveram-se nas camadas superficiais do solo no período chuvoso e aprofundaram-se nos períodos de deficiência hídrica. No entanto, os adultos demonstraram menor tolerância ao estresse hídrico e aprofundaram-se logo no início da época seca, enquanto as ninfas o fizeram somente no final desse período. Os resultados obtidos nesse trabalho são semelhantes aos verificados para outras espécies de Scaptocoris, em outras regiões.

Studies on population dynamics and vertical distribution in the soil of Scaptocoris carvalhoi Becker were carried out in a pasture area in Paraúna, State of Goias, Brazil, from November 2004 to October 2005. One meter-deep holes were excavated in the soil, except on the season of lower precipitation, when 2-meter holes were excavated (June, August and September 2005). On each evaluation, the specimens were collected and grouped according to the depth they were found in the soil profile (0-20, 21-40, 41-60, 61-80 and 81-100 cm). Nymphs occurred during the entire length of the study and were more abundant than adults. Adult population augmented with the increase of rainfall. Nymphs and adults were located in the top layer of the soil during the rainy season and were found deeper in the soil during droughts. However, adults were less tolerant to hydric stress and burrowed deeper into the soil at the beginning of the dry season, while nymphs burrowed deeper only at the end of the season. Results of this study are in accordance to other studies on various species of Scaptocoris, in other regions of Brazil.